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kinE [2018-02-11T09:12:24.000Z]
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kinE [2018-02-11T09:12:24.000Z]

two-component sensor kinase, phosphorylates Spo0F, part of the phosphorelay
locus
BSU13530
pI
5.49
mw
85.32 kDa
protein length
738 aa Sequence Blast
gene length
2214 bp Sequence Blast
function
initiation of sporulation
product
two-component sensor kinase
essential
no
synonyms
ykrQ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.2|Control of two-component response regulators] → [category|SW 3.4.2.2.1|Two-component sensor kinase]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.7|phosphorelay] → [category|SW 3.4.7.1|The kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.2|phosphorelay] → [category|SW 4.2.2.1|The kinases]

    • Gene

    Coordinates
    1,419,213 → 1,421,429

    The protein

    Catalyzed reaction/ biological activity

  • autophosphorylation, phosphorylation of [protein|9896B99346B0D3F6D57F57377DB253B46135A37A|Spo0F]

    • Paralogous protein(s)

  • [protein|4EE48E4931F662E51586DB6D01E91C688329222D|CssS], [protein|9E8A6AB3920BFBFDAAD1F2E4F333A32354ABB25B|YkoH], [protein|52BB660B0CAB36C74F1D47963235846B45AF78AB|YclK], [protein|96E4470EF4B558F4EC318FEBF58B779BA50DB7E2|YvrG]

    • [SW|Domains]

  • two transmembrane segments
  • [SW|PAS domain]
  • C-terminal histidine phosphotransferase domain

    • Modification

  • autophosphorylation on a His residue

    • Expression and Regulation

    Operons

    genes
    [gene|1582E81F7DA85F38D6732D341ABD0F052587F25A|kinE]-[gene|9492D341F54BE019B21C0BAF7465AD8F84CC6F23|ogt]
    description
    [Pubmed|2506524]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2506524], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
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    Biological materials

    Mutant

  • MGNA-B319 (ykrQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1318 NBRP B. subtilis, Japan]
  • BKE13530 (Δ[gene|1582E81F7DA85F38D6732D341ABD0F052587F25A|kinE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE13530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCATGTACACTCCTTCT, downstream forward: _UP4_GAATGAACTACTATACGACA
  • BKK13530 (Δ[gene|1582E81F7DA85F38D6732D341ABD0F052587F25A|kinE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK13530 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCATGTACACTCCTTCT, downstream forward: _UP4_GAATGAACTACTATACGACA

    • References

  • 10094672,11069677,2506524